rabbit anti ap2β  (Novus Biologicals)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Single-Cell Characterization of the Frizzled 5 ( Fz5 ) Mutant Mouse and Human Persistent Fetal Vasculature (PFV)

doi: 10.1167/iovs.64.3.8

Figure Lengend Snippet: Expression of neural crest markers Sox10 and AP2β in the vitreous. Throughout the figure panels, Sox10 is in red , and AP2β is in green . “+/+”, wild-type mice ( Fz5 +/+ ); “−/−”, mutant mice ( Fz5 −/− ). ( A–D ) Sox10 and AP2β expression at E11.5. Arrows point to the Sox10 positive cells. ( E–H ) E15 eye sections. Arrows point to the forming hyaloid vitreous. Noting more cells in the mutant vitreous at either E11.5 or E15. ( I–M ) P3 retina. Arrows point to the retrolental mass. ( M ) is a magnified boxed area in ( L ). ( N–R ) P6 retina. Arrows point to the retrolental mass. ( R ) is a magnified boxed area in ( Q ), which is horizontally orientated.

Article Snippet: The primary antibodies used for this study were rabbit anti-Col1a1 (1:500, A1352; ABclonal Technology, Woburn, MA, USA), rat anti-CD31 (1:500, 557355; BD Bioscience), rabbit anti- AP2β (1:200, 2509S; Cell Signaling Technology, Danvers, MA, USA), mouse anti-alkaline phosphatase (1:100, 14-9870-82; Invitrogen, Carlsbad, CA, USA), rabbit anti-Pax3 (1:100, bs-1097R; Bioss Antibodies Inc., Woburn, MA, USA), goat anti-Sox10 (1:20, AF2864; R&D Systems, Minneapolis, MN, USA), and mouse anti-Tyrp1 (1:100, ab3312; Abcam, Cambridge, MA, USA).

Techniques: Expressing, Mutagenesis

Journal: Investigative Ophthalmology & Visual Science

Article Title: Single-Cell Characterization of the Frizzled 5 ( Fz5 ) Mutant Mouse and Human Persistent Fetal Vasculature (PFV)

doi: 10.1167/iovs.64.3.8

Figure Lengend Snippet: Astroglia were present in the Fz5 mutant vitreous as indicated by GFAP. Throughout panels, GFAP is in red , and AP2β is in green . “+/+”, wild-type mice ( Fz5 +/+ ); “−/−”, mutant mice ( Fz5 −/− ). ( A–H ) P3 retina. ( A, B ) Bright-field microscopy. Arrows in ( B ), ( D ), and ( F ) point to the pigmentary retrolental mass. Arrowheads in ( F ) point to the vitreous hyaloid vessel fragments positive for GFAP in the mutants. ( I–P ) Same panel arrangement as in ( A–H ).

Article Snippet: The primary antibodies used for this study were rabbit anti-Col1a1 (1:500, A1352; ABclonal Technology, Woburn, MA, USA), rat anti-CD31 (1:500, 557355; BD Bioscience), rabbit anti- AP2β (1:200, 2509S; Cell Signaling Technology, Danvers, MA, USA), mouse anti-alkaline phosphatase (1:100, 14-9870-82; Invitrogen, Carlsbad, CA, USA), rabbit anti-Pax3 (1:100, bs-1097R; Bioss Antibodies Inc., Woburn, MA, USA), goat anti-Sox10 (1:20, AF2864; R&D Systems, Minneapolis, MN, USA), and mouse anti-Tyrp1 (1:100, ab3312; Abcam, Cambridge, MA, USA).

Techniques: Mutagenesis, Microscopy